Aktivitas Antioksidan Fraksi Etil Asetat dan Fraksi Air Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight.) Walp.) dengan Metode DPPH (1,1 Difenil- 2 pikrilhidrazil)

Susilowati Susilowati; Sari Wulandari

Authors

Susilowati Susilowati Sekolah Tinggi Ilmu Kesehatan Nasional
Sari Wulandari Sekolah Tinggi Ilmu Kesehatan Nasional

Keywords:

daun salam, antioksidan, fraksi etil asetat, fraksi air, DPPH, IC50

Abstract

Salam plants are one of the plants that are often used by the community for alternative medicine. The part of the greeting plant commonly used as a treatment is the leaves. Bay leaves contain flavonoids, alkaloids, terpenoids / steroids, and tannins. The content of flavonoids contained in bay leaves can act as natural antioxidants. This study aims to determine the potential of antioxidant activity of ethyl acetate fraction and bay leaf water fraction. Fractionation is done in stages using n-hexane, ethyl acetate, and water solvents. The ethyl acetate fraction and water fraction obtained were tested for its compound content using phytochemical examination. The antioxidant activity of ethyl acetate fraction and water fraction was carried out using the DPPH method. The results of phytochemical examination of ethyl acetate fraction and water fraction contain flavonoids, alkaloids, tannins, and terpenoids. The results of the measurement of antioxidant activity of bay leaf ethyl acetate fraction has the potential to be very strong with an IC50 value of 47.7709 ppm and the potential fraction of bay leaf water with a IC50 value of 52.3957 ppm.

References

Agustina, S., Ruslan, R., dan Wiraningtyas, A., (2016), Skrining Fitokimia Tanaman Obat Di Kabupaten Bima, CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry), 4(1), 71-76.

Artini, P., Astuti K., dan Wardiatiani, N., (2013), Uji Fitokimia Ekstrak Etil Asetat Rimpang Bengle (Zingiber purpureum Roxb.), Jurnal Farmasi Udayana. 2 (4), 1-7

Bahriul, P., Rahman, N., dan Diah, A.W.M., 2014, Uji Aktivitas Ekstrak Daun Salam (Syzygium polyanthum) dengan Menggunakan 1,1-Difenil-2-Pikrilhidrazil, Jurnal Akademika Kimia, 3(3): 143-149.

Dewi, A.S., 2007, Uji Antioksidan Fraksi Etil Asetat dan Fraksi Air Ekstrak Etanol Teh Hijau Melalui Penangkapan Radikal Hidroksil dengan Metode Deoksiribosa, Skripsi, Fakultas Farmasi Universitas Sanata Dharma, Yogyakarta.

Harismah, K., 2017, Pemanfaatan Daun Salam (Eugenia Polyantha) Sebagai Obat Herbal Dan Rempah Penyedap Makanan, Warta LPM, 19(2): 110-118.

Hasanah, N., 2015, Aktivitas Antioksidan Ekstrak Etanol Daun Salam, Jurnal Pena Medika, 5(1): 55-59.

Hikmah, N., Yuliet, Y., dan Khaerati, K., 2016, Pengaruh Pemberian Ekstrak Daun Salam (Syzygium Polyanthum Wight.) Terhadap Glibenklamid Dalam Menurunkan Kadar Glukosa Darah Mencit (Mus Musculus) Yang Diinduksi Aloksan, Jurnal Farmasi Galenika (Galenika Journal of Pharmacy), 2(1): 24-30.

Jun, M.H.Y., Yu, J., Fong, X., Wan, C.S., dan Yang, C.T., 2003, Comparison of Antioxidant Activities of Isoflavones from Kudzu Root (Pueraria labata Ohwl), Journal Food Science, Institute of Technologist, 68(6): 2117-2122.

Kaempe, H.S., Suryanto, E., dan Kawengian, S.E., 2013, Potensi Ekstrak Fenolik Buah Pisang Goroho (Musa Spp.) terhadap Gula Darah Tikus Putih (Rattus norvegicus), CHEMISTRY PROGRESS, 6(1): 6-9.

Nurrachmawati, I., 2017, Efek Ekstrak Daun Salam (Syzygium polyanthum) Terhadap, Glukosa Darah Sewaktu, Kadar Profil Kolesterol dan Diabetik Kardiomiopati pada Tikus Diabetes Melitus, Bachelor's thesis, Fakultas Kedokteran dan Ilmu Kesehatan UIN Syarif Hidayatullah, Jakarta.

Ritna A., Syaiful A., dan Akhmad K., 2016, Identiikasi Senyawa Flavonoid pada Fraksi Etil Asetat Benalu Batu (Begonia sp.) Asal Kabupaten Morowali Utara, Galenika Journal of Pharmacy, 2(2): 83-89.

Robinson, T., 1995, Kandungan Organik Tumbuhan Tinggi Edisi VI, Terjemahan Kosasih Padmawinata, Fakultas Matematika dan Ilmu Pengetahuan Alam Institut Teknologi Bandung, Bandung.

Saifudin, A., Viesa, R., dan Hilwan, Y.T., 2011, Standarisasi Bahan Obat Alam, Graha Ilmu, Yogyakarta.

Sangi M., Lidya M., dan Maureen K., 2012, Uji Toksisitas dan Skrining FitokimiamTepung Gabah Pelepah Aren (Arenga pinnata), Jurnal Ilmiah Sains, 12(2): 127-134.

Saparinto, C., dan Rini S., 2015, Panduan Praktis Menanam 28 Tanaman Bumbu Dapur Populer di Pekarangan, Lily Publisher, Yogyakarta.